Figure 6

Involvement of Pyk2 and FAK in MSU-induced inflammatory responses in vivo.

(A) ELISA of IL-1β in the supernatant of mouse BMDMs primed for 4 h with LPS and then stimulated with ATP (for 4 h) or nigericin (for 1 h) in the presence or absence of PF-562271. All results are presented as the mean ± SD of three independent experiments, and were analyzed using the Student’s t test. (B) Schematic presentation of MSU-induced peritonitis in mice. (C) ELISA of IL-1β in the peritonea of mice treated for 6 h with intraperitoneal injection of MSU in the presence or absence of PF-562271. (D) Absolute number of peritoneal exudate cells (PECs), CD11b⁺/Ly6G⁺/F4/80⁻ neutrophils, and CD11b⁺/F4/80⁺ monocytes-macrophages in the peritonea of mice treated for 6 h with intraperitoneal injection of MSU in the presence or absence of PF-562271. Each symbol represents an individual mouse (n = 3 per group); small horizontal lines indicate the mean (±s.d.). Symbol: *P < 0.05.