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Figure 1

From: Sulforaphane, a natural component of broccoli, inhibits vestibular schwannoma growth in vitro and in vivo

Figure 1

Sulforaphane inhibits proliferation of primary human vestibular schwannoma (VS) cells in a dose-dependent manner.

(A) Proliferation of primary human VS cells derived from a combination of 3 different VSs, as measured using bromodeoxyuridine (BrdU) incorporation (red), was inhibited by application of sulforaphane (10 μM) for 48 h. BrdU incorporation was calculated as the percentage of BrdU-labeled nuclei relative to total nuclei stained with Hoechst33342. Scale bar = 100 μM (same scale bar applies to all). Bar graph depicts mean ± standard error of mean (SEM, n = 9). ***p < 0.001. (B) Cell viability (measured with MTT assay) of human HEI-193 cells and (C) mouse SC4 Nf2−/− Schwann cells incubated with 5–25 μM concentrations of sulforaphane for 48 h. Bar graphs represent mean ± SEM (n = 6).

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