Figure 3
From: TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna
Genotypes of transgenic lines (TG1–TG3).
A complete schematic is shown for each of the three transgenic lines produced in this study (TG1, TG2, and TG3). The top image shows the complete structure of the endogenous locus into which donor DNA was integrated. The structure of the integrated plasmid in the genome, and sequences of the junctions between transgene and the surrounding genome are schematically represented at the middle and bottom, respectively. NcoI sites used for inverse PCR are shown above each gene. Primer binding sites for inverse PCR are shown with red arrows. Primer positions for genomic PCR to determine 5′ and 3′ junction sequences of the transgene are indicated with black and grey arrows. Red, blue, and grey boxes, and green arrows indicate the ey1 TALEN target, the ey2 TALEN target, the JHRE element, and the H2B-GFP reporter gene, respectively. Arrowheads show the ey1 TALEN and ey2 TALEN cleavage sites.
