Figure 3
Colony formation assay (A) and BrdU label retention assay (B) for human OMECs isolated by dispase II/trypsin-EDTA (D/T) or collagenase (Col). (A) More colonies (first row, A) and larger colonies (second row, A) were formed from OMECs isolated by collagenase, indicating a higher proportion of progenitor cells (*p < 0.05). Moreover, higher p75NTR expression was observed in the collagenase-isolated group (p75, green; bottom row, A; *p < 0.05). (B) Retained BrdU labeling (green) after additional 2 weeks of culture was significantly increased in the collagenase-isolated group (first and second rows, (B); *p < 0.05). Moreover, higher p63 expression (red) was observed in the collagenase-isolated group (third row, B; nuclei were counterstained with Hoechst 33342; *p < 0.05). (C) Western blotting for p75NTR, TAp63, and ΔNp63 expressed by OMECs isolated by dispase II/trypsin-EDTA or by collagenase.
