Figure 7
Oral supplementation with KB reversed abnormal locomotor rhythmicity of ApoE−/− mice.
(a) Representative actograph showing the wheel-running activities under the DD condition of ApoE−/− mice fed with regular chow, then switched to regular chow supplemented with 1.5% followed by 3% KB each for 7 days and finally switched back to regular chow (the start of the treatment indicated by open and solid arrows for 1.5% and 3% KB respectively, and the end of the treatment indicated by arrowhead). (b) Representative actograph showing the wheel-running activities under the dLD condition of ApoE−/− mice fed with regular chow, then switched to regular chow supplemented with 3% KB for 7 days, and finally switched back to regular chow (the start of the treatment indicated by solid arrow and the end of the treatment indicated by arrowhead). (c) Representative actograph showing the wheel-running activities under the dLD condition of ApoE−/− mice fed with regular chow, then switched to regular chow supplemented with 1.5% KB for 7 days, and finally switched back to regular chow (the start of the treatment indicated by open arrow and the end of the treatment indicated by arrowhead). Note that 1.5% KB supplementation partially reversed, while 3% KB supplementation completely reversed the free-running arrhythmic running, and this effect lasted for 18 days after KB withdrawal. (d,e) Locomotor activity in the light and dark phases and light/dark ratio under the dLD condition of ApoE−/− mice fed with regular chow supplemented with 3% KB (n = 6 for both ApoE−/− and C57BL/6J mice, ** indicates P < 0.01, *indicates P < 0.05).
