Figure 2
(A,B) Adsorption isotherms of DNA on sepiolite in the presence of various polyvalent cations. Each point has error bars for 3 different experiments. Reaction conditions: 10 mM Tris-HCl pH = 7.5 and a sepiolite concentration of 1 mg/ml. (C) The effect of the presence of cations with different valences on DNA adsorption. Reaction conditions: 10 mM Tris-HCl pH = 7.5, salmon sperm DNA and sepiolite concentrations of 615 ng·μl−1 and 1 mg/ml, respectively. (D,E) Adsorption isotherms of different DNA conformations on sepiolite. Reaction conditions: 10 mM Tris-HCl pH = 7.5, 5 mM MgCl2, sepiolite concentration of 1 mg/ml; 50 μg of sepiolite was used in each experiment. Adsorption occurred at 25 °C for 24 hours under agitation at 700 rpm using an Eppendorf Thermomixer.
