Figure 6 | Scientific Reports

Figure 6

From: Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells

Figure 6

(A) Delivery of fluorescent siRNA by sepiolite in A673 human sarcoma cells. The cells are treated for 3 h alone (A673 cells) or with 50 nM FITC labeled siRNA (green) free or complexes to sepiolite at mass ratio of 10 or 25 (Sepiolite/siRNA; weight/weight). Cell nuclei are stained by DAPI (blue). Scale bar is 20 μm. (B) Efficiency of siRNA delivery by Sepiolite in A673 human sarcoma cells. After 3 h incubation, A673 sarcoma cells are fixed and observed by epifluorescence microscopy and the percentage of cell containing green fluorescent siRNA is determined on different slide fields for each one conditions of treatment.

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