Figure 2
From: Identification of suitable reference gene and biomarkers of serum miRNAs for osteoporosis
Identified miR-25-3p as the suitable reference gene for osteoporosis.
(a) Ranking of candidate reference genes by geNorm, the most stable pair was recommended as miR-25-3p and miR-342-5p. (b) Ranking of candidate reference genes by NormFinder. (c) The expression pattern of candidate gene during hindlimb-unloading (HU) within 6 weeks, miR-25-3p showed as the most stable gene. *p < 0.05, HU. VS. CN, n = 6, N = 72. (d) miR-25-3p was detected in the serum of Sham and OVX rats (n = 12). (e) Rhesus monkeys with (n = 6) or without bedrest (n = 9). (f) Postmenopausal women with normal BMD (n = 19) or with osteopenic (n = 7) or with osteoporosis (n = 10). (g) miR-25-3p in the femur of hindlimb-unloading for 4 W (n = 6). (h) miR-25-3p in the femur of OVX for 8 W (n = 6). (i) miR-25-3p in BMMs after osteoclastic induction for 4d (n = 3). (j) miR-25-3p in primary osteoblasts after osteogenic induction for 0d, 3d, 7d, 14d and 21d (n = 4). All these showed no significant differences (N.S.) (p > 0.05). BR: Bedrest group, Con: control group.
