Table 3 Descriptive statistics for oxidative markers measured in liver tissue of lactating and non-reproductive mice selected for high and low food intake.

From: Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance

Variables

NH

RH

NL

RL

Line

Reproductive status

n

PC

0.64 ± 0.03

0.62 ± 0.03

0.68 ± 0.03

0.66 ± 0.05

F1,37 = 1.87, P = 0.180

F1,37 = 0.29,P = 0.592

40

8OHdG*

57.0 ± 12.90

76.10 ± 14.50

70.80 ± 15.20

63.80 ± 13.70

F1,18 = 0.01, P=0.990

F1,18 = 0.22, P = 0.647

21

8OHdG†

2.84 ± 0.44

1.57 0.24

2.16 ± 0.68

4.70 ± 1.03

F1,21 = 0.98, P = 0.333

F1,21 = 0.90, P = 0.354

24

SOD

12.11 ± 1.46

14.12 ± 0.83

14.19 ± 0.65

12.99± 0.97

F1,37 = 0.20, P = 0.654

F1,37 = 0.20, P = 0.710

40

CAT

15.27a ± 0.81

10.48b ± 0.87

12.44a,b ± 1.29

10.83b ± 0.60

F1,37=1.70, P = 0.210

F1,37 = 11.30, P = 0.002

40

GPx

612.70a,b ± 65.10

286.10c ± 24.09

727.50a ± 29.80

506.20b ± 23.10

F1,35 = 18.50, P < 0.001

F1,35 = 49.60, P < 0.001

38

  1. Measurementsof various oxidative stress markers in liver tissue of reproductive (R) and non-reproductive (N) mice of selection lines for high (H) or low (L) food intakes.Values shown are mean ± SEM. Results of two-way ANOVAs are shown with line and reproductive status as fixed factors. No significant interactions between line and reproductive status were found in the models and these were therefore removed before final analysis. NH: Non-reproducing high food intake mice; RH: Reproducing high food intake mice; NL: Non-reproducing low food intake mice; RL: Reproducing low food intake mice. PC: Protein carbonyls; 8OHdG: 8-hydroxy-2-deoxyguanosine; *measured by ELISA method; †measured by HPLC method; SOD: Superoxide dismutase; CAT: Catalase; GPx: Glutathione peroxidase. Different letters indicate significant differences between groups (post-hoc Tukey tests, p < 0.05).
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