Figure 3
From: Epac1 interacts with importin β1 and controls neurite outgrowth independently of cAMP and Rap1
Epac1 inhibits neurite outgrowth, which is exacerbated in the absence of impβ1.
(A) N2A cells overexpressing YFP-Epac1 or GFP, were cultured in serum-free medium (SFM; solid bars) to induce differentiation or kept in regular growth medium (RM; open bars). Cells were also treated with impβ1 si-RNA (si-impβ1) or control si-RNA (si-ctl). Neurite length was analyzed after 24 hrs as described in the methods section. Data shown are the mean ± SEM neurite lengths for three independent experiments where at least 4 images of >40 cells each were analyzed for each condition. Data were analyzed by Two-Way ANOVA; ***P < 0.0001. (B) Representative images of cells from each condition cultured in serum free medium; YFP-Epac1 (green), βIII-tubulin (red), and DAPI (blue); scale bar corresponds to 25 μm.
