Table 3 Primers for real-time fluorescence quantitative PCR.

From: Children’s Caregivers and Public Playgrounds: Potential Reservoirs of Infection of Hand-foot-and-mouth Disease

Virus Name

Primer Name

Sequence 5′–3′*

3′ Label

5′ Label

Size (bp)

Gene Target

Enterovirus 36

EV-Forward primer

CCCTGAATGCGGCTAATCC

    
 

EV-Reverse primer

ATTGTCACCATAAGCAGCCA

    
 

EV-Probe

AACCGACTACTTTGGGTGTCCGTGTTTC

BHQ1

FAM

146

Polyprotein

CV-A16 37

CV-A16-Forward primer

CCTACAGCTGCCAACACTGAGG

    
 

CV-A16-Reverse primer

CATTAGACGACGCCCCTGTCTC

    
 

CV-A16-Probe

CAYAGATTAGGCACTGGTGTTGTACCAGCA

BHQ1

FAM

94

VP1

EV71 37

EV71 -Forward primer

TTCATGTCACCYGCGAGYGC

    
 

EV71-Reverse primer

GCYCCRTATTCAAGRTCTTTCTC

    
 

EV71-Probe

TAYGACGGRTAYCCCACRTTYGGWGA

BHQ1

FAM

92

VP1

CV-A6 38

CV-A6-Forward primer

CAAGCTGCAGAAACGGGAG

    
 

CV-A6-Reverse primer

GCTCCACACTCGCCTCATT

    
 

CV-A6-Probe

ACCCCGTTTCGATTCATCACACA

BHQ1

FAM

103

VP1

  1. *R = A or G; Y = C or T; W = A or T.
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