Figure 4 | Scientific Reports

Figure 4

From: Transcriptome Analysis of the Signalling Networks in Coronatine-Induced Secondary Laticifer Differentiation from Vascular Cambia in Rubber Trees

Figure 4

The qRT-PCR analysis of JAZ family members at different time intervals after COR and water treatments.

The epicormic shoots were treated with either 20 μM COR or water. Cambia-containing tissues were collected at one hour (1 h), two hours (2 h), four hours (4 h), eight hours (8 h), one day (1 d), two days (2 d) and three days (3 d) after treatment. The relative expression of each gene was calculated as the 2−ΔΔCt value and normalized to the endogenous reference gene, HbUBC2a. SD of three biological replicates is indicated by 1, 2 or 3 asterisks depending on the P value for the significant difference (P < 0.05, P < 0.01, P < 0.001, respectively) as determined by a one-way ANOVA.

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