Figure 5
mTORC1 inhibitior rapamycin and ER stressor tunicamycin induce different patterns of ER-mitochondria Ca2+ transfer.
(A) Representative confocal fluorescence images of control HeLa cells (con) or cells treated with tunicamycin (tun) or rapamycin (rap), loaded with Rhod-FF before (upper panels) and after stimulation with histamine 10 μM for 100 s (middle panels). Differences between these images are shown in pseudocolors (lower panels). Scale bars = 10 μm. (B) Peak fluorescence intensity of mitochondrial Ca2+ signals imaged as in (A) calculated locally according to radial analysis (n = 3). (C) Representative curves of mitochondrial Ca2+ signals imaged as in (A) depicting signal differences between regions of the radial analysis. Data are shown as the mean ± SEM. *P ≤ 0.05 compared with controls (con).
