Figure 6
mTORC1 inhibitor rapamycin and ER stressor tunicamycin generate mitochondria with different functional profiles.
(A) Representative confocal fluorescence images of control HeLa cells (con) or cells treated with tunicamycin (tun) or rapamycin (rap), stained with MitoTracker Orange (upper panels) and mtHsp70 (middle panels). Ratios between these images are shown in pseudocolors (lower panels). Scale bars = 10 μm. (B) Global fluorescence ratios between MitoTracker Orange (MTO) and mtHsp70 signals imaged as in (A) (n = 3). (C) MitoTracker Orange (MTO) and mtHsp70 fluorescence ratios of images obtained as in (A) calculated locally according to radial analysis, normalized to the sum of ratios across all regions (n = 3). Data are shown as the mean ± SEM. *P ≤ 0.05 compared with controls (con). #P ≤ 0.05 comparing tunicamycin (tun) and rapamycin (rap).
