Figure 2
The changes of autophagy during wound healing.
(a) Skin tissues from the control animals that wounded only were harvested for beclin-1 detection using IHC with beclin-1 antibody. The positive cells are brown as indicated by black arrows. Scale bars = 100 μm. (b) The average scores of beclin-1 staining were evaluated (4 groups, 6 time points as indicated, n = 6 per group per time point). Data is presented as the mean ± SD. *P < 0.05, **P < 0.01. (c) Electron microscopy was employed to observe numbers of autophagosome and autolysosome in wounds. Arrows indicate autophagosome or autolysosome. Scale bars = 500 nm. (d) The LC3 staining was sperformed in wounds with rapamycin or 3-MA treatment. Brown staining represents the positive cells as indicated by black arrows. Scale bars = 100 μm. (e) The average scores of LC3 staining were evaluated at different time points as indicated (n = 6 per group per time point). Data is presented as the mean ± SD. *P < 0.05.
