Figure 3
The changes of autophagy in impaired diabetic wounds healing.
(a) A full-thickness skin defects was made on each db/db or db/m mice. The db/db mice were pre-treated with or without 3-MA (10 mg/kg/d) by i.p. from 1 day before wounding and up to the wounds closed completely. Representative photographs of wounds closure are presented. (b) The areas of wounds were quantified and the ratios of wounds closure were expressed (3 groups, n = 10 per group). Data is presented as the mean ± SD. *P < 0.05, **P < 0.01. (c) The LC3 staining was performed using IHC. The positive cells are brown as indicated by black arrows. LC3 positive cells in wounds in db/db mice are compared to that in db/m mice. Scale bars = 100 μm. (d) The average scores of LC3 staining were evaluated (two groups, 6 time points as indicated, n = 6 per group per time point). Data is presented as the mean ± SD. *P < 0.05. (e) Immunofluorescence was employed to determine LC3 positive expressions. Green staining represents the positive cells as indicated by white arrows, blue staining represents nucleus. Scale bars = 100 μm.
