Figure 6
The effects of AGEs on macrophages.
(a) RAW264.7 cells were treated with AGEs (100 μg/ml) for 24 h, numbers of autophagosome and autolysosome were observed using electron microscopy. Scales bar = 500 nm. (b) RAW264.7 cells were treated with AGEs, 3-MA (2 mM), and AGEs plus 3-MA for 24 h, respectively. CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. LC3 protein bands were obtained using western blotting. (c) After treating with AGEs, 3-MA, or AGEs plus 3-MA for 24 h, the cells were stained with LC3 antibody using immunofluorescence. Representative photographs were taken under Laser Scanning Confocal Microscope. The LC3 positive cells (red) are presented, blue staining represents nucleus. Scale bars = 10 μm.
