Figure 6
Impact of GAG derivatives on TNAP activity.
hBMSC were cultured on TCPS (CTRL) and treated with dissolved GAG derivatives as described. TNAP activity was determined from cell lysates at day 8. Diagrams show calculated values as mean ± SEM. (A) hBMSC were treated with 200 μg GAG derivative/mL for 8 days. Diagram shows TNAP activities calculated from four different donors and untreated control (CTRL) was set to 100%. Significant differences between sHA1 and CTRL, HA or Hep are indicated with c (p < 0.001) and b (p < 0.01). (B) hBMSC were treated with 200 μg HA or sHA1/mL or with the equivalent ATTO565-labeled derivative for 8 days. n = 3. (C) hBMSC were exposed to 200 μg sHA1/mL for different time frames (d1-8, d4-8, d7-8) and TNAP activities were determined at day 8. Untreated control (CTRL) was set to 100%. Significant differences between (d1-8) and CTRL, (d4-8) or (d7-8) are indicated with c (p < 0.001) and a (p < 0.05). n = 3.
