Figure 1 | Scientific Reports

Figure 1

From: Remote in vivo stress assessment of aquatic animals with microencapsulated biomarkers for environmental monitoring

Figure 1

(a) General scheme of the preparation of MBMs based on the pH-sensitive fluorescent dye SNARF-1 (here, only 3 layers of positively charged polymer, 3 layers of negatively charged polymer and a final coating of biocompatible polymer are depicted). (b) Image of the prepared MBMs recorded using an epifluorescent microscope. (c) Calibration curve of the pH-sensitive MBMs at different pHs (mean ± s.d. is depicted). (d) E. verrucosus, with the main body segments, central haemolymph vessel and point of injection of MBMs indicated. (e) System used for amphipod immobilisation under the epifluorescent microscope.

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