Figure 3

DCs migrating on immobilized CCL21 24-98 bio.
(a) DCs migrating in a microfluidic channel treated with SA-Cy3 and CCL21 24-98 bio without B4F laser-writing. (b) DCs migrating on a SA-Cy3 stained CCL21 24-98 bio patch printed in a microfluidic channel. (c) DCs migrating on a SA-Cy3 stained CCL21 24-98 bio gradient printed in a microfluidic channel. Left panels: Representative images of the SA-Cy3 stained patterns. Middle panel: Trajectories of all cells in the respective field of view, with trajectories plotted to a common starting point [dimensions in μm; time is colour-coded]. Gradient direction and pattern shape are indicated in grey. Right panel: Rose plot visualizing the distribution of angles of all cell tracks in an angular sector field with tracks split into 3 min intervals.