Figure 4
From: The prion protein family member Shadoo induces spontaneous ionic currents in cultured cells
Sho protein, akin to PrPΔCR induces inward ionic currents.
(a) Representative traces of whole cell patch clamp recordings on SH-SY5Y cells non-transfected or expressing the indicated proteins: wild-type or mutant Sho or PrP, or the empty vectors (possessing EGFP or mCherry expression reporter) at holding potential of −80 mV. (b) Current activity recorded in the different types of SH-SY5Y cell clones, plotted as percentage of total time the cells exhibited inward currents ≥200 pA. (Mean ± SEM; SH-SY5Y: no currents detected; GFP: no currents detected; mCherry: 0.3 ± 0.1; PrP∆CR: 45.4 ± 9.4; Sho + mCherry: 14.9 ± 4.9; Sho + GFP: 18.5 ± 0.1; PrP: no currents detected; Sho + PrP: 2.0 ± 0.01; 307: 0.46 ± 0.01; 309–250: 0.54 ± 0.0; 324–200: 11.67 ± 0.05). In brackets the number of samples for each cell type is indicated. Of note, in approximately 25% of PrP∆CR or Sho cells, recordings lasted less than 2 min. These data, which may reflect an intrinsic cell fragility, were omitted from the analyses.
