Figure 5
From: The prion protein family member Shadoo induces spontaneous ionic currents in cultured cells
Sho protein expressed in HEK293 cell line induces inward ionic currents.
(a) Representative traces of whole cell patch clamp recordings on HEK293 cells expressing mCherry only or the wild-type Sho, as well as co-expressing Sho and GFP or PrP at holding potential of −80 mV. (b) Current activity recorded in the different types of HEK293 cells, plotted as percentage of total time the cells exhibited inward currents ≥200 pA. (Mean ± SEM; mCherry: no currents detected; Sho: 17.8 ± 4.1; Sho + GFP: 22.0 ± 6.4; Sho + PrP: 4.4 ± 2.4). The number of recorded cells for each type is indicated in brackets.
