Figure 4
iSEC1 is expressed by a small fraction of CD45− epithelial cells in the gastrointestinal tract.
(a) NIH3T3 transfectants expressing mock control, iSEC1, iSEC2, or CD200 were stained with GT42 or anti-CD200 (OX90) mAb (open histograms), or isotype matched control antibody (shaded histograms). (b) Cells isolated from indicated gastrointestinal tissues were stained with anti-CD45 in combination with anti-iSEC1 (GT42), anti-CD200 (OX90) or isotype-matched control. (c) CD45− cells isolated from the jejunum epithelium were analyzed for the expression of iSEC1 in combination with CD326, CD66a or CD24. (d) Cells isolated from the jejunum epithelium were incubated with CD200R-IgFc or control FLAG-IgFc fusion proteins, followed by detection of bound fusion proteins with anti-IgG1 in combination with CD326 and CD45 staining. CD45− cells were gated for analysis shown here. Data shown in (a–d) are representative of three independent experiments.
