Figure 1 | Scientific Reports

Figure 1

From: Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy

Figure 1

PC-3 spheroid formed in an EZSPHERE 3D culture plate and different cell density in the 2D culture.

All cells were incubated for 4 days. (a) Phase-contrast images of a spheroid. Scale bar, 100 μm. (b) Hoechst 33342 stains for nuclei. A z-stack section was obtained every 2 μm. Scale bar, 20 μm. (c) The diameter of formed spheroids. S125, 1.25; S250, 2.50; S500, 5.00 × 105 cells/35-mm dish. Over 40 spheroids were counted. (d) Phase-contrast images of cells in the 2D culture. Scale bar, 500 μm. (e) Cellular protein amount measured by Bradford assay; n = 3. All bars represent standard deviation (SD).

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