Figure 2

CD4⁺CXCR5^hiPD-1^hi and CD4⁺CXCR5^hiICOS^hi Tfh cells are preferentially accumulated in the UP.

(a–d) Representative flow cytometric plots and cumulative data illustrating the percentages of CXCR5^hiPD-1^hi (a,c) and CXCR5^hiICOS^hi (b,d) populations among CD4⁺ T cells in the PB, spleen, thymus, BM and UP of maternal mice on E11.5. Each symbol reflects the data from a single mouse (n ≥ 6 mice per group) and the data are representative of two independent experiments. The cells are gated in CD4⁺ T cells. Data were assessed statistically using the Kruskal-Wallis test followed by Dunns multiple-comparison test. PB: peripheral blood; BM: bone marrow; UP: uterus and placenta; hi: high; **p < 0.01; ***p < 0.001.