Figure 6
Effects of PDL1 blockade on foetal resorption, and on the proportion, cellularity and maturation of maternal CD4+ T cells.
Adult BALB/c mice were mated with C57BL/6 mice, and the pregnant females were then injected intraperitoneally with anti-mouse PDL1 blocking mAb at a dose of 250 μg on E5.5 and E8.5, respectively. Both the control and PDL1-blocked mice were sacrificed on E11.5. (a) Representative diagrams illustrate the whole uterus (containing embryos and placentas) of the control and PDL1-blocked mice, and resorbed embryos are indicated by arrows. (b) Resorption rate. (c,d) Representative flow cytometric plots (c) and cumulative data (d) illustrating the percentage and absolute number of CD4+ T cells in the maternal spleen. (e,f) Representative flow cytometric plots illustrating the percentage of CD4+ T cells in the maternal BM (e) and UP (f). (g–i) Representative flow cytometric plots (g), bar graphs (h) and pie charts (i) displaying the percentages of indicated cells in the maternal thymus from the control and PDL1-blocked mice. (j) The absolute number of indicated cells in the maternal thymus. Each symbol reflects the data from a single mouse (n ≥ 8 mice per group) and the data are combined from four independent experiments. The cells are gated from lymphocytes. The data were assessed statistically using the Mann-Whitney U test (a) or unpaired Student’s t-test (d,h,j). BM: bone marrow; UP: uterus and placenta; No.: number; ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001.
