Figure 1
From: Infliximab and Dexamethasone Attenuate the Ductular Reaction in Mice
Histological evaluation of CDE- and DDC-treated livers.
(A) Livers retrieved from unchallenged or mice-fed with a CDE or DDC diet for 14 days. Left lobes are divided for immunohistochemistry studies via embedding-paraffin processing. (B) Liver sections were stained with hematoxylin/eosin and CK19. After 7 days, CDE-treated livers accumulate fat (red arrows), which becomes more pronounced after 2 weeks of treatment, and small oval cells are appearing progressively, whereas porphyrin crystals appearing as brown pigments (red asterix) and stratification of bile ducts were the only striking features observed in livers of DDC-treated mice. In CDE livers, CK19+-cells are dramatically increased in number, forming arborescence inside the lobule. Those cells are organized in small clusters (1), or duct-like structures (2), or isolated (3) far from the PV area. DDC induces morphological alterations of the bile ducts (flattening of cholangiocytes, thickened multi-layered bile duct walls delineating a pseudo lumen) (yellow arrows). All images were taken in 20x original magnification.
