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Figure 1

From: Surface-bound bovine serum albumin carrier protein as present in recombinant cytokine preparations amplifies T helper 17 cell polarization

Figure 1

Effect of coated versus soluble fractalkine preparations on TH17 cell polarization in CX3CR1−/− cells.

(a–c) Wildtype and fractalkine receptor deficient (CX3CR1−/−) cells were subjected to TH17 polarization with IL-6, TGFβ and IL-23 in the absence and presence of coated (“coat”) and soluble (“sol”) recombinant fractalkine at 20 and 100 nM final concentrations. The proportion of TH17 cells was assessed by intracellular IL-17A staining after re-stimulation on day 4 (a, examples, b statistical analysis of fractalkine from Peprotech, and c, from R&D Systems, n = 4–8 from 2–4 indep. exp.).

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