Figure 1
From: Retargeted Foamy Virus Vectors Integrate Less Frequently Near Proto-oncogenes
Modified FV Pol and Gag helper plasmids for retargeted integration into heterochromatin.
(a) Schematic representations of the normal and modified Pol constructs which encode the viral protease (PR), reverse transcriptase (RT), and integrase (IN) proteins. The modified Pol construct expresses chromobox protein homolog 1 (CBX1) fused to IN (IN-CBX1) to retarget integration. (b) Schematic representations of the normal and modified Gag constructs. The modified Gag construct expresses a chromatin binding site (CBS) domain with a triple alanine substitution of RTY (Gag-RTY). The triple alanine mutation reduces the binding affinity of CBS with host chromatin. CMV, cytomegalovirus promoter; WT, wild type.
