Figure 3
The effect of DSPE-PEG on the CLA-PTX NPs particle size.
(A) The effect of CLA-PTX concentration and added volume as well as DSPE-PEG on the particle size of CLA-PTX NPs. A volume of 0.3 ml of a series of CLA-PTX mixed DSPE-PEG (the ratio of CLA-PTX: DSPE-PEG = 1:0.1, w/w) DMSO solution added to 3 ml distilled water (blue line and blue solid diamond); A volume of 1 ml of a series of CLA-PTX mixed DSPE-PEG (the ratio of CLA-PTX: DSPE-PEG = 1:0.1, w/w) DMSO solution added to 3 ml distilled water (blue line and blue empty diamonds). The particle size of CLA-PTX NPs was determined after dialysis against distilled water for 48 h (MWCO = 3500 Da). (B) The effect of the added amounts of DSPE-PEG on the CLA-PTX NPs particle size. The ratio of CLA-PTX:DSPE-PEG was set at 1:0.0125, 1:0.025, 1:0.05, 1:0.1, 1:0.15, 1:0.2, 1:0.33, 1:1, 1:3, 1:5, 1:7, 1:10, 1:20, 1:40 or 1:80 (w/w). The concentration of CLA-PTX was 2 mg/ml. According to the CLA-PTX NPs preparation, a volume of 0.3 ml CLA-PTX mixed DSPE-PEG DMSO solution was added drop-wise to 3 ml distilled water under continuous and gentle stirring (300–500 rpm) at room temperature. As a control group, the CLA-PTX was replaced by PTX. Similarly, the ratio of PTX:DSPE-PEG was set at 1:0.0125, 1:0.025, 1:0.05, 1:0.1, 1:0.15, 1:0.2, 1:0.33, 1:1, 1:3, 1:5, 1:7, 1:10, 1:20, 1:40 or 1:80 (w/w). The concentration of PTX was 2 mg/ml. According to the CLA-PTX NPs preparation, a volume of 0.3 ml of PTX mixed DSPE-PEG DMSO solution was added drop-wise to 3 ml distilled water under continuous and gentle stirring (300–500 rpm) at room temperature.
