Figure 2

Splenic cell population analysis of pre-diseased Sharpin^cpdm mice demonstrates cellular dysregulation.

Representative images of control and Sharpin^cpdm mice on day 45 post birth (a) and day 25 post birth (d), depicting the extent of dermatitis on the dorsal and ventral sides. White dotted line outlines the area of dermatitis in the Sharpin^cpdm mice. Spleen image (b,e) and weight (c,f) of control and Sharpin^cpdm mice on indicated days. (g–m) Flow cytometry analysis of splenocytes from control and Sharpin^cpdm mice on day 25. Representative flow plots of CD11b⁺Gr1⁺ neutrophils (g), CD4⁺ and CD8⁺ T cells (i), and CD19⁺MHCII⁺ B cells (l) in the spleen. Cumulative bar graphs representing frequencies of CD11b⁺Gr1⁺ neutrophils (h), CD4⁺ T cells (j), CD8⁺ T cells (k), and CD19⁺MHCII⁺ B cells (m) in the spleen. Control, n = 7; Sharpin^cpdm, n = 6 for (c). Control, n = 4; Sharpin^cpdm, n = 4 for h, j, k, and m. Bar graphs are presented as means ± s.e.m. and are representative of at least two independent experiments. Statistical significance was determined by Mann-Whitney testing, and P values less than 0.05 are considered statistically significant. *P < 0.05.