Figure 5

IL-1α is dispensable for induction of disease in Sharpin^cpdm mice.

(a) Control (n = 11), Sharpin^cpdm (n = 10), and Sharpin^cpdm × Il1a^−⁄− (n = 10) mice were followed after weaning and scored for the onset of dermatitis. Mice that showed any sign of skin inflammation were scored as disease-positive and indicated in the disease score curves on that day. (b) Representative images of control, Sharpin^cpdm, and Sharpin^cpdm × Il1a^−⁄− mice on day 45 post birth, depicting the extent of dermatitis on the dorsal and ventral sides. White dotted line outlines the area of dermatitis in Sharpin^cpdm and Sharpin^cpdm × Il1a^−⁄− mice. (c–d) Spleen harvested from control, Sharpin^cpdm and Sharpin^cpdm × Il1a^−⁄− mice on day 45. Representative images (c) and spleen weight (d) of control, Sharpin^cpdm, and Sharpin^cpdm × Il1a^−⁄− mice. (e–k) Flow cytometry analysis of splenocytes from control, Sharpin^cpdm, and Sharpin^cpdm × Il1a^−⁄− on day 45. Representative flow plots of CD11b⁺Gr1⁺ neutrophils (e) CD4⁺ and CD8⁺ T cells (g) and CD19⁺MHCII⁺ B cells (j) in the spleen. Cumulative bar graphs representing frequencies of CD11b⁺Gr1⁺ neutrophils (f) CD4⁺ T cells (h) CD8⁺ T cells (i) and CD19⁺MHCII⁺ B cells (k) in the spleen. Control, n = 6; Sharpin^cpdm, n = 6; Sharpin^cpdm × Il1a^−⁄−, n = 4 for d, f, h, i, and k. Disease curve in (a) was analyzed by log rank (Mantel-Cox) testing. Bar graphs are presented as means ± s.e.m. Statistical significance between groups was determined by one-way ANOVA followed Dunnett’s multiple comparisons testing, and P values less than 0.05 are considered statistically significant. ns = not significant, *P < 0.05, **P < 0.01.