Table 1 Correspondence between genetic markers on LG2, positive BAC clones identified from BAC pools, sequenced BAC clones selected based on MTPs/FPC contigs of the Asian seabass physical map and assembled scaffolds by using Miseq alone as well as Miseq-PacBio hybrid assembly.
From: BAC-pool sequencing and analysis confirms growth-associated QTLs in the Asian seabass genome
Marker | Genbank Accession No. | Relative position (cM) | No. of positive clones detected | FPC contig | No. of selected BACs on MTP | Expected size (kb) | MiSeq | MiSeq + PacBio | ||
|---|---|---|---|---|---|---|---|---|---|---|
No. of scaffolds | Assembly size (kb) | No. of scaffolds | Assembly size (kb) | |||||||
LcaE169 | HQ233685 | 0 | 2 | ctg2943 | 2 | 100.6 | 15 | 120.8 | 4 | 164.7 |
Lca342 | DQ290189 | 8.6 | 8 | ctg654 | 4 | 418.9 | 46 | 560.3 | 12 | 653.4 |
LcaTe0354 | HQ233689 | 10.3 | 3 | ctg157 | 3 | 420.2 | 65 | 415.8 | 25 | 743.8 |
LcaTe0360 | HQ233690 | 15.2 | 2 | ctg462 | 3 | 375.4 | 46 | 235.5 | 1 | 271.5 |
Lca182 | DQ290150 | 18.4 | 6 | ctg1122 | 5 | 495.0 | 18 | 403.8 | 11 | 713.6 |
Lca287 | DQ290155 | 24.4 | 3 | ctg1327 | 7 | 587.5 | 23 | 593.4 | 12 | 894.4 |
Lca276 | DQ290147 | 28.7 | 2 | Singleton | 1 | 108.8 | 4 | 84.9 | 3 | 150.8 |
Lca250 | DQ290127 | 34.6 | 4 | ctg1474 | 1 | 151.0 | 10 | 127.6 | 3 | 194.8 |
Lca964 | HQ233694 | 37.3 | 2 | ctg564 | 3 | 284.2 | 36 | 264.9 | 11 | 387.4 |
LcaTe0359 | HQ233696 | 41.1 | 3 | ctg1597 | 2 | 182.2 | 24 | 241.4 | 7 | 402.6 |
LcaTe0138 | HQ233697 | 42.7 | 3 | ctg2977 | 3 | 307.4 | 12 | 189.4 | 7 | 378.3 |
Lca562 | HQ233699 | 44.9 | 2 | ctg381 | 5 | 493.7 | 33 | 299.1 | 11 | 541.4 |
Lca418 | DQ431148 | 48.1 | 1 | ctg1727 | 2 | 182.2 | 17 | 145.0 | 7 | 360.5 |
Lca480 | HQ233700 | 50.3 | 7 | ctg1876 | 5 | 420.2 | 58 | 196.2 | 13 | 445.3 |
Lca524 | HQ233701 | 51.3 | 2 | ctg2518 | 3 | 198.6 | 5 | 148.4 | 4 | 347.2 |
LcaE92 | HQ233705 | 54.4 | 2 | ctg3569 | 2 | 258.4 | 13 | 186.4 | 8 | 359.7 |
LcaTe0447 | HQ233706 | 55.4 | 4 | ctg2781 | 2 | 146.9 | 7 | 254.9 | 3 | 246.3 |
Lca371 | DQ290210 | 61.3 | 5 | ctg710 | 5 | 480.1 | 63 | 461.1 | 19 | 784.2 |
LcaTe0533 | HQ337090 | 64 | 7 | ctg1634 | 3 | 243.4 | 11 | 203.1 | 7 | 371.5 |
LcaE186 | HQ337091 | 75.7 | 8 | ctg1200 | 6 | 488.2 | 39 | 332.0 | 6 | 465.9 |
LcaTe0605 | HQ337094 | 81.4 | 3 | ctg1654 | 2 | 168.6 | 12 | 146.6 | 3 | 369.1 |
LcaB128 | EU072400 | 97.5 | 7 | ctg677 | 3 | 529.0 | 47 | 269.8 | 10 | 480.5 |
Total | / | / | 86 | / | 72 | 7,040.7 | 604 | 5,880.3 | 187 | 9,726.6 |
