Table 1 Characteristics of TMP-mutant derivatives of TP901-1.
From: Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1
TP901-1 mutant | #aa deleted | Tail length (nm) | E.O.P. | Plaque morphology | γFrequency of lysogeny (FL) | FL upon addition of tails |
|---|---|---|---|---|---|---|
TP901-1erm WT | 0 | 118 | 1 | Clear, 1.5 mm | 4.4 × 10−3 | 4.8 × 10−3 |
Δ1–2 | 29 | 109.5 | 4.5 | Clear, 1.5 mm | 3.8 × 10−3 | ND |
Δ1–3 | 40 | 6.8 × 10−2 | 0.75 mm | 1.4 × 10−3 | ND | |
Δ1–4 | 51 | ≤1.5 × 10−8 | NA | 9.0 × 10−5 | ND | |
Δ1–5 | 69 | 3 | Clear, 1 mm | 1.8 × 10−3 | ND | |
Δ1–7.5 | 100 | 101 | ≤1.5 × 10−8 | NA | 1.1 × 10−4 | 2.3 × 10−3 |
Δ1–8 | 109 | 3 | 0.75 mm | 5.3 × 10−3 | ND | |
Δ1–9 | 120 | 98 | 0.88 | Clear, 1 mm | 4.5 × 10−3 | ND |
Δ1–10 | 131 | 6.8 × 10−4 | 0.5 mm | 3.5 × 10−3 | ND | |
Δ1–12 | 153 | 6 | Clear, 1.5 mm | 1.9 × 10−3 | ND | |
Δ1–15 | 186 | 5.4 | Clear, 1.5 mm | 2.6 × 10−3 | ND | |
Δ1–18 | 219 | 1.4 | Clear, 1.5 mm | 7.6 × 10−3 | ND | |
Δ1–29 | 347 | 65 | ≤1.5 × 10−8 | NA | 1.6 × 10−5 | 6.5 × 10−4 |
Δ20–26 | 84 | 6.5 | Clear, 1.5 mm | 2.2 × 10−3 | ND | |
Δ24–26 | 40 | 4.3 | Clear, 1.5 mm | 2.4 × 10−3 | ND | |
Δ24–29 | 73 | 2.0 × 10−3 | Fuzzy, 1 mm | 3.5 × 10−3 | ND | |
Δ1–5, 24–26 | 109 | 0.6 | 0.5 mm | 4.2 × 10−3 | ND | |
Δ1–9, 24–26 | 160 | 8.2 × 10−3 | 0.5 mm | 1.7 × 10−3 | ND | |
Δ1–9, 20–26 | 204 | 86.7 | 0.34 | 0.5 mm | 2.4 × 10−3 | ND |
Δ1–9, 24–29 | 193 | ≤1.5 × 10−8 | NA | 4.3 × 10−5 | 3.5 × 10−3 | |
Δ1–9, 20−29 | 237 | ≤1.5 × 10−8 | NA | 1 × 10−4 | 2.1 × 10−3 | |
Δ1–12, 20–29 | 270 | 77 | ≤1.5 × 10−8 | NA | 4.6 × 10−4 | 1.0 × 10−3 |
Δ1–15, 20–29 | 303 | ≤1.5× 10−8 | NA | 3.4 × 10−4 | 1.5 × 10−3 | |
Δ1–18, 20–29 | 336 | ≤1.5× 10−8 | NA | 3.4 × 10−4 | ND | |
ΔTM1-3 | 71 | ≤1.5 × 10−8 | NA | ≤1.5 × 10−8 | 2.8 × 10−3 | |
ΔTM3 | 19 | ≤1.5 × 10−8 | NA | 3.2 × 10−4 | 2.0 × 10−3 | |
ΔTM2-3 | 43 | 107.6 | ≤1.5 × 10−8 | NA | 1.3 × 10−7 | 2.6 × 10−3 |
ΔTM4-6 | 89 | 6.8 × 10−2 | NA | 4.4 × 10−3 | ND | |
ΔTM1-6 | 160 | No tails | ≤1.5 × 10−8 | NA | ≤1.5 × 10−8 | 4.0 × 10−3 |
ΔTM4 | 23 | 3.4 | Clear, 1.5 mm | 8.9 × 10−4 | ND | |
ΔE2-F31 | 30 | No tails | ≤1.5 × 10−8 | NA | ≤1.5 × 10−8 | 3.1 × 10−3 |
ΔT908-F937 | 30 | No tails | ≤1.5× 10−8 | Clear, 1.5 mm | ≤1.5 × 10−8 | 2.7 × 10−3 |
ΔF810-G842 | 33 | 9.5 × 10−7 | pinpoint | 4.4 × 10−4 | ND | |
ΔL843 –V875 | 33 | 1.4 × 10−5 | pinpoint | 7.4 × 10−5 | ND | |
ΔF810-V875 | 66 | 2.0 × 10−6 | pinpoint | 5.4 × 10−5 | ND | |
Δ1–29, F810-G842 | 380 | ≤1.5 × 10−8 | NA | 4.1 × 10−5 | 2.8 × 10−3 | |
Δ1–29, F810-V875 | 413 | ≤1.5 × 10−8 | NA | 3.4 × 10−6 | 1.9 × 10−3 | |
Δ1–29, F810-T908 | 446 | No tails | ≤1.5 × 10−8 | NA | ≤1.5 × 10−8 | 3.5 × 10−3 |
Δ F31-I61 | 30 | ≤1.5× 10−8 | NA | ≤1.5 × 10−8 | 6.0 × 10−4 | |
Δ F31-L141 | 110 | No tails | ≤1.5 × 10−8 | NA | ≤1.5 × 10−8 | 5.8 × 10−4 |
Δ I62-L141 | 80 | ≤1.5 × 10−8 | NA | ≤1.5 × 10−8 | 3.4 × 10−3 | |
ΔA142-E154 | 13 | ≤1.5 × 10−8 | NA | ≤1.5 × 10−8 | 2.2 × 10−3 | |
Δ I62- L141, Δ1–29, F810-T908 | 526 | ≤1.5 × 10−8 | NA | ≤1.5 × 10−8 | 2.6 × 10−3 |
