Figure 4
Solamargine inhibited mRNA and promoter activity of MUC1 through activation of AMPK.
(A) PC3 and DU145 cells were exposed to solamargine (6 μM) for 24 h, followed by measuring the mRNA expressions of MUC1 by qRT-PCR. (B) PC3 and DU145 cells were transfected with wild type human MUC1 promoter reporter construct ligated to luciferase reporter gene and internal control secreted alkaline phosphatase for 24 h, followed by treating with solamargine for an additional 24 h. (C) PC3 and DU145 cells were treated with compound C (10 μM) for 2 h before transfecting the cells with wild type human MUC1 promoter reporter construct ligated to luciferase reporter gene and internal control secreted alkaline phosphatase for 24 h, followed by treating with solamargine (6 μM) for an additional 24 h. Afterwards, the promoter activities were determined using the Secrete-Pair Dual Luminescence Assay Kit as described in the Materials and Methods Section. *Indicates significant difference as compared to the untreated control group (P < 0.05). **Indicates significant difference from solamargine treated alone (P < 0.05).
