Figure 1 | Scientific Reports

Figure 1

From: 3-D EM exploration of the hepatic microarchitecture – lessons learned from large-volume in situ serial sectioning

Figure 1

Comparative overview of the five experimental conditions assessed for their suitability for the application of SBF-SEM.

Column 1 provides a global overview of rat liver tissue, revealing large polyhedral parenchymal cells (PC), separated by the hepatic sinusoids (S) that are lined by liver sinusoidal endothelial cells (LSEC). Kupffer cells (Kc) can also be visualised within the lumen of the sinusoids. Column 2 reveals notable variations in the presence, preservation quality and degree of contrasting of a range of subcellular structures, including mitochondria (m), rough endoplasmic reticulum (rER), lipid droplets (l), glycogen (g) and nucleoli (n). Column 3 corresponds to the 3-D reconstructed data of the five experimental conditions examined. Reconstructed 3-D volumes consist of 450 consecutive images (section thickness = 200 nm). Dimensions of 3-D volumes: XY = 122.61 μm Z = 90 μm. Total volume = 1,350,055.23 μm3. Scale bar = 20 μm.

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