Figure 3
Three-dimensional reconstruction and corresponding model views of the hepatic microarchitecture prepared under the NCMIR protocol and imaged utilising SBF-SEM.
(A) Reconstructed 3-D volume consisting of 500 consecutive images (section thickness = 200 nm). XY = 122.61 μm Z = 100 μm. Total volume = 1,503,394.77 μm3. (B) 2-D serial-section showing parenchymal cells (multi-coloured) arranged in cords, which have been segmented by means of manual tracing using IMOD. Modelled parenchymal cells (C,D,F) are formed via the superimposition of numerous segmented lines. Hepatic sinusoids (white spaces surrounding parenchymal cells) were segmented by means of isosurface thresholding. (C) Model view of partial and entire parenchymal cells (multi-coloured) and the hepatic sinusoids (red). (D) Model view of whole parenchymal cells (multi-coloured) surrounded by the tortuous hepatic sinusoids (red). (E) Model view of the hepatic sinusoids (red) within the reconstructed 3-D volume, which were segmented by means of isosurface/thresholding segmentation. (F) Model view of whole parenchymal cells (multi-coloured) within the reconstructed 3-D volume. Magnification = 796x Scale bar = 50 μm.
