Figure 7 | Scientific Reports

Figure 7

From: 3-D EM exploration of the hepatic microarchitecture – lessons learned from large-volume in situ serial sectioning

Figure 7

Stages of specimen mounting for SBF-SEM.

(A) Exposed surface of resin-embedded rat liver tissue visible along the sectioned block face (white trapezoid). (B) Corresponding semithin section (0.5 µm-thick) showing a large vein (black arrowhead) surrounded by anastomosing sinusoids (white spaces). (C). The precision trimmed block has been centred and adhered to the specimen pin (top view) using cyanoacrylate glue. The exposed edges of the tissue were pained with silver paint, after which the entire block was gold coated in order to assist with alignment of the specimen within the SBF-SEM. (D) Longitudinal view of the specimen pin with gold-coated sample mounted atop (black arrowhead) in preparation for SBF-SEM imaging. Scale bar (A–C) = 500 μm, (D) = 5 mm.

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