Table 2 Summary of optimised imaging conditions to minimise charging and maximise brightness and contrast for each respective specimen preparation protocol.

From: 3-D EM exploration of the hepatic microarchitecture – lessons learned from large-volume in situ serial sectioning

 

SCF

GOT

TAMOI

ROUM

NCMIR

Accelerating voltage (kV)

1.4

1.5

1.5

1.4

3.5

 

5

10

9

7

28

Objective aperture size (μm)

60

60

60

30

30

Back to article page