Figure 5
From: An iron detection system determines bacterial swarming initiation and biofilm formation
ICDH-Coumarin produced from pvc gene cluster chelates Fe3+ and blocks iron binding to RssA.
(a) LC-MS chromatogram of ethyl acetate extracts from WT, Δpvc, ΔrssBA, and ΔrssBA-pvc bacteria harboring pBAD33 (vector) or pPvc encoding pvc cluster under DMM broth with arabinose (0.3%). *Indicates the peak and structure of ICDH-Coumarin as determined by NMR. (b) Fe3+ chelation activity of DFO, ICDH-Coumarin, and 2,2′-DP at the indicated concentration. (c) His-tagged periplasmic domain of RssA was incubated with 55FeCl3 and distilled water (mock), DFO (0.3 mM), ICDH-Coumarin (0.3 mM), or 2,2′-DP (0.3 mM). Radioactivity was determined from the eluted periplasmic domain. One-way ANOVA with *** corresponding to P-value < 0.001 compared to mock group.
