Figure 5

Oral administration of 2-AB leads to elevated 2-AB as well as GSH in plasma and hearts, and exerts a cardioprotective effect.

(a) Body weight of 2-AB treated mice after 1 week oral administration of 2-AB. Bars indicate the mean ± s.d. (n = 5, control and 1 mM 2-AB treated mice; n = 4, 10 mM 2-AB treated mice). (b,c) The levels of 2-AB in plasma (b) (n = 4, control, n = 5, 1 mM 2-AB treated mice, n = 4, 10 mM 2-AB treated mice) and hearts (c) (n = 5, control and 1 mM 2-AB treated mice; n = 4, 10 mM 2-AB treated mice) with GC-MS analysis. (d,e) The levels of GSH in plasma (d) and hearts (e) (n = 4, control, n = 5, 1 mM 2-AB treated mice and 10 mM 2-AB treated mice). (f) Chronic doxorubicin (DOX)-induced cardiomyopathy was induced with 10 mg/kg intraperitoneal injection (i.p.) of DOX as shown. Before the DOX injection, mice were administrated orally for 14 days with the vehicle solution or 10 mM 2-AB solution. (g) Fractional shortening (FS) as assessed by echocardiography 6 days after the initial DOX injection. (h) The levels of GSH in hearts in DOX-injected mice (n = 4, each group). PO, per ou. Bars indicate the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.0001.