Figure 1 | Scientific Reports

Figure 1

From: Activated hepatic stellate cells play pivotal roles in hepatocellular carcinoma cell chemoresistance and migration in multicellular tumor spheroids

Figure 1

Human hepatic stellate cells (HSCs; LX2) enhance the compactness of loosely aggregated spheroids from HCC cell lines and primary HCC cells.

(A) HepG2 and PLC/PRF/5 cells, which formed loosely aggregated spheroids alone, were co-cultured with stromal cells (LX2, WI38, and HUVECs) at a 1:1 ratio for 3 days (upper panel). To calculate the volume of spheroids, their long and short diameter were examined (lower panel). (B) 118965, which is a primary HCC cell line that formed loosely aggregated spheroids alone, were co-cultured with LX2, WI38, and HUVEC cells at a 1:1 ratio for 3 days (upper panel). Volume of spheroids were analyzed (lower panel). All bright field images of spheroids were obtained using the Operetta® High Content Screening System with a 10× objective. Data are shown as means ± SD from three independent experiments with triplicate. **P < 0.005. Scale bar = 200 μm.

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