Figure 3 | Scientific Reports

Figure 3

From: Activated hepatic stellate cells play pivotal roles in hepatocellular carcinoma cell chemoresistance and migration in multicellular tumor spheroids

Figure 3

HSCs, which are cultured with conditioned media (CM) from 3D HCC cells, exhibit a myofibroblast-like phenotype.

(A) Schematic of the CM experiment in (B,C). Huh7 cells were cultured under 2D and 3D condition using the same number of cells with the same amount of media. After 3 days, the CM was collected and used to treat LX2 cells for 48 and 72 hr. (B) LX2 cells were treated with complete media and CM from 2D and 3D culture for 48 hr. The phenotype of the cells was examined using bright field images. (C) LX2 cells that were treated with complete media and CM from the 2D and 3D HCC cells were collected at the indicated times (0, 48 and 72 hr). LX2 lysates were analyzed by western blotting using anti-α-SMA and anti-β-actin (control) antibodies. Values were normalized to β-actin. Data are shown as means ± SD from three independent experiments. Scale bar = 500 μm.

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