Figure 4 | Scientific Reports

Figure 4

From: Activated hepatic stellate cells play pivotal roles in hepatocellular carcinoma cell chemoresistance and migration in multicellular tumor spheroids

Figure 4

Collagen 1A1 expression is increased by the interaction between HSCs and HCC cells in MCTS.

HepG2 and PLC/PRF/5 spheroids were cultured alone or with LX2, WI38, and HUVEC stromal cells at 1:1 ratio for 3 days. After 3 days, mRNA expression in each spheroid was evaluated by (A) RT-PCR and (B) real-time PCR using collagen and non-collagen-related ECM primers. Values were normalized to GAPDH. Data are shown as means ± SD from two independent experiments with duplicates.

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