Figure 4 | Scientific Reports

Figure 4

From: Genomic and transcriptomic profiling of resistant CEM/ADR-5000 and sensitive CCRF-CEM leukaemia cells for unravelling the full complexity of multi-factorial multidrug resistance

Figure 4

mFISH analysis of CCRF-CEM and CEM/ADR5000 cells.

Two clones detected in CCRF-CEM are depicted in (A,B). All derivative chromosomes present in clones 1 and 2 are highlighted by light-green arrows. Individual changes for clones 1 and 2 are labelled by arrows in darker green. For derivative chromosome 5, a whole chromosome paint (wcp) and a subtelomeric (st) probe for 5qter were applied. For the derivative chromosome 8, a centromeric probe (D8Z1) and a st probe for 14qter had been used. In (4C) to F, CEM/ADR5000 clones 1, 1a, 1b, 1b1 and 1c are depicted. The per clone acquired alterations are highlighted by coloured arrows as explained in the legend between (4A/B) and C/D. For clear visualisation of the inversion in chromosome 7, MCB 7 was applied as shown in (4C). In (4C), the only additional aberration present in clone 1a is depicted, i.e. a reciprocal translocation between chromosomes 6 and 14.

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