Figure 1
From: Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile
CRISPR/Cas9-mediated generation of Prm2-deficient mice.
(a) Schematic representation of the Prm2 gene locus and targeting sites of designed gRNAs. (b) Surveyor Assay. Intended target sites of gRNAs were amplified by PCR from genomic DNA of gene-edited E14TG2a ES cells. Wildtype and gene-edited PCR products were denaturated and re-annealed. Surveyor nuclease mediated cleavage of mismatched PCR products (highlighted by arrowheads) confirmed functionality of gRNAs. (c,d) Genotyping of offsprings by PCR and Surveyor assay identified 4/10 animals to be mutant as indicated by asterisks (PCR) and pluses (Surveyor).
