Figure 2
From: Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile
Knockout validation and fertility analysis.
(a) Schematic representation of established Prm2-deficient mouse lines Prm2Δ97bp and Prm2Δ101bp. Blue triangle marks cleavage site within the premature PRM2 protein. (b) Mating statistics of Prm2-deficient males and females. Successful mating of Prm2−/− males with wildtype females was indicated by presence of a vaginal plug at 0.5 dpc. (c) Relative expression of Prm2 mRNA in murine testis of wildtype, Prm2+/−, and Prm2−/− mice measured by qRT-PCR. (d) Immunohistochemical staining of PRM2 on testicular sections of wildtype, Prm2+/− and Prm2−/− mice. Scale bar = 100 μm. (e) Immunoblot against PRM2 following acid urea gel electrophoresis of basic nuclear proteins isolated from epididymal sperm of Prm2Δ97bp or Prm2Δ101bp mice. Of note, the amount of protein loaded from Prm2-deficient samples were doubled compared to samples from wildtype and heterozygous animals. Coomassie-Brilliant-Blue staining (CBB) served as loading control. The antibody detects mature PRM2 as well as its precursor forms (pP2-a, pP2-b).
