Figure 5
From: Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile
Effects of Prm2-deficiency on histone-to-protamine exchange dynamics.
(a) Immunoblot against H3 and TNP1 of basic nuclear proteins of epididymal sperm and testis from Prm2+/+, +/− and −/− mice (b) Immunohistochemical staining of total H3, TNP1 and PRM1 on testicular sections from Prm2Δ mice. Sections were counterstained with hematoxylin. Tubules with elongating spermatids were marked with a star, tubules with mature spermatids with arrowheads. Scale bar = 100 μm. (c) Relative expression of Prm1 mRNA in murine testis of wildtype, Prm2+/− and Prm2−/− mice measured by qRT-PCR (n = 3). (d) Immunoblot against PRM1 following acid urea gel electrophoresis of basic nuclear proteins isolated from epididymal sperm of Prm2+/+, Prm2+/−, and Prm2−/− mice. Reanalysis of blots shown in Fig. 2e.
