Figure 1 | Scientific Reports

Figure 1

From: Identification of novel MYO18A interaction partners required for myoblast adhesion and muscle integrity

Figure 1

Biochemical interaction between MYO18A and its partners.

Co-immunoprecipitation following transfection of different expression vectors in HEK293T cells. (A) Schematic representation of full-length proteins and related expression constructs. Known functional domains (solid black or grey boxes) are shown, with abbreviations indicated on the top and in the text. (B) EGFP-tagged MYO18A PDZ domain is expressed in different conditions (upper panel) and immunoprecipitated (middle panel) by myc-tagged p190RhoGEF or Lurap1, which are detected by western blot (arrowhead and arrow in lower panel, respectively). (C) EGFP-tagged Golgin45 is expressed in different conditions (upper panel) and immunoprecipitated (middle panel) by myc-tagged full-length MYO18A, which is detected by western blot (lower panel).

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