Figure 1

Cardiac progenitor cells phenotype differ between β-blocker and non-β-blocker patients.

Representative cell culture images and corresponding CS yield quantification (a) show how β-blocker (BB) patients were significantly more efficient in giving CSs compared to non β-blocker (NBB) patients (when considering only the few successful NBB explants) (n = 3 biological replicates). Consistently with the CS-formation process being TGFβ-dependent, BB CPCs have significantly higher expression levels of TGFBR2 (b) (n = 3–7). The immunophenotypes of BB and NBB-CDCs were significantly different in the abundance of the CD90 + subpopulation (c) (n = 4–9), also shown by the representative flow cytometry histograms (d). BB and NBB CDCs also significantly differed in their expression levels of collagen I (COL-A1) and III (COL3) (e) (n = 3–7), and in their expression ratio (f). ^*P < 0.05. ^**P < 0.01. Scale bars = 250 μm.